2-10
Preparing System Hardware
Priming the binary solvent manager
Priming, a timed operation, replaces solvent in the path from the reservoirs to
the vent valve, not including the flow control module. During priming, the
vent valve moves to Vent position to ensure minimal backpressure. The flow
rate during priming is 8 mL/min total (4 mL/min each for pumps A and B).
Prime the binary solvent manager when performing these tasks:
•
Changing reservoirs or solvents
•
Preparing a new system or binary solvent manager for use
•
Running the system after it has been idle for more than four hours
Caution:
To prevent salts from precipitating in the system, introduce an
intermediate solvent, such as water, when changing from buffers to
high-organic-content solvents. For details, see the nanoACQUITY UPLC
System Operator’s Guide.
Recommendations:
•
Whenever you change solvents, always purge and autozero the flow
control module (see the nanoACQUITY UPLC System Operator’s Guide).
•
Ensure the solvent reservoirs contain enough solvent for adequate
priming and use, and ensure the waste container has sufficient capacity
for used solvent. The priming flow rate is 4 mL/min for each pump, or
8 mL/min total, so priming both solvents for 5 minutes requires
approximately 20 mL of each solvent.
To prime the binary solvent manager:
1.
In the nanoACQUITY UPLC Console, select Binary Solvent Manager
from the system tree.
2.
Click Control > Prime A/B Solvents.
Summary of Contents for nanoACQUITY UPLC
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