Preparing the detector
2-21
Preparing the detector
If your system includes a TUV detector or mass spectrometer, prepare it for
operation by following the procedures in this section.
See also:
the documentation included with your mass spectrometer.
Preparing the TUV detector
Preparing the TUV detector involves two steps: starting and verifying.
Starting the detector
Use only thoroughly degassed HPLC-grade solvents. Gas in the mobile phase
can form bubbles in the flow cell, causing the detector to fail the power-up
diagnostic tests.
To start the TUV detector:
1.
Ensure the detector flow cell is filled with transparent solvent (methanol
or water) that it is free of air bubbles, and that the door is closed firmly.
Tip:
The detector might not initialize correctly if the flow cell contains
air.
2.
Press the power switch on the detector door. The detector beeps three
times and runs a series of startup tests while the lamp LED blinks.
Initialization requires approximately 2 minutes, and lamp warm-up
requires approximately 3 minutes.
3.
When the lamp LED shows constant green, start MassLynx. You can
monitor the nanoACQUITY UPLC Console for messages and visual
signals. For best results, allow 30 minutes for the baseline to stabilize.
Tip:
The absorbance value appears in the nanoACQUITY UPLC Console
and also in the MassLynx Inlet Editor window. If the detector is in dual
wavelength mode, two absorbance values appear.
Absorbance values have a resolution of 0.0001 AU.
When the lamp is extinguished, “Lamp Off” appears instead of
absorbance values.
4.
Configure the detector according to the instructions in the MassLynx
Online Help.
Summary of Contents for nanoACQUITY UPLC
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