2-8
Preparing System Hardware
Preparing the binary solvent manager
For optimal performance of the nanoACQUITY UPLC system, you must
prepare the solvent manager for operation. Preparing the solvent manager
includes these tasks:
•
Priming the seal wash
•
Priming the binary solvent manager
Requirements:
•
To maintain the efficiency of the binary solvent manager, and to obtain
accurate, reproducible chromatograms, use only HPLC-grade (or higher)
quality solvents, water, and additives. For details, see the
nanoACQUITY UPLC System Operator’s Guide.
•
Solvent A must be aqueous and solvent B must be organic (acetonitrile
or methanol).
•
The flow control module supports only the solvents shown in the Solvent
drop-down list of the solvent manager’s instrument method dialog box.
Priming the seal wash
Prime the seal wash in the binary solvent manager to lubricate the plungers
and flush away solvent and/or any precipitated salts that have seeped past the
plunger seals from the high-pressure side of the piston chambers.
Prime the plunger seal wash under these conditions:
•
After using buffered mobile phase
•
When the binary solvent manager has been inactive for a few hours or
longer
•
When the binary solvent manager is dry
•
When troubleshooting a low-pressure error
Caution:
To avoid damage to the solenoid valve seats and seals in the solvent
path, do not use a nonvolatile buffer as the weak wash or strong wash solvent.
Rule:
To prevent contamination, do not recycle seal wash.
Warning:
Observe safe laboratory practices when you handle solvents.
See the Material Safety Data Sheets for the solvents you use.
Summary of Contents for nanoACQUITY UPLC
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