4-2
Verifying System Operation
Requirement:
To prevent contamination, always use powder-free nitrile
gloves when handling components of the nanoACQUITY system.
Caution:
Never change directly between immiscible eluents or between
buffered solutions and organic eluents. Immiscible eluents form emulsions in
the flow path. Combining buffered solutions and organic eluents can result in
salt precipitation in the gradient proportioning valves, pump heads, check
valves, or other parts of the system. Confirm that all fluids in the system are
miscible with acetonitrile.
Preparing the mobile phases
The verification test requires two mobile phases: 0.1% formic acid/water for
solvent A and 0.1% formic acid/acetonitrile for solvent B. The test also
requires weak wash and strong wash.
Requirement:
All solvents must be HPLC-grade or better.
To prepare solvent A, 0.1% formic acid/water:
1.
Measure 100 mL of HPLC-grade water in a 100-mL graduated cylinder.
2.
Carefully transfer the water to a 250-mL reservoir bottle.
3.
Pipette 100
µ
L of formic acid into the reservoir bottle.
4.
Cap the reservoir bottle and mix well.
5.
Label the reservoir bottle as 0.1% formic acid/water.
6.
Submerge lines A1, Seal Wash, Weak Wash, and Strong Wash in the
solvent A reservoir bottle with 0.1% formic acid/water.
7.
Place the solvent A reservoir bottle in the solvent tray.
To prepare solvent B, 0.1% formic acid/acetonitrile:
1.
Measure 100 mL of acetonitrile in a 100-mL graduated cylinder.
2.
Carefully transfer the acetonitrile to a 250-mL reservoir bottle.
3.
Pipette 100
µ
L of formic acid into the reservoir bottle.
4.
Cap the reservoir bottle and mix well.
5.
Label the reservoir bottle as 0.1% formic acid/acetonitrile.
Summary of Contents for nanoACQUITY UPLC
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