2-16
Preparing System Hardware
Tip:
Priming the system washes the sample needle, so whenever you prime
the system, you can omit this procedure.
Recommendations:
•
Do not use buffered solvents as wash solvents.
•
Match the types of organic species in the wash solvents to those in the
chromatographic application.
Example:
If the weak wash solvent is 30
%
acetonitrile and 70
%
water,
the strong wash solvent should contain a greater concentration of
acetonitrile in water.
•
To ensure that the strong wash solvent is completely removed, the
system washes the needle with 200
µ
L of weak wash solvent after you
use strong wash solvent. You can increase, but not decrease, the default
value of 200
µ
L.
•
Do not interrupt the priming sequence; wait until it finishes.
Before you begin, ensure that the solvents are compatible with your
application, that their volumes are sufficient, and that the waste reservoir is
large enough to contain the waste solvent.
To wash the sample manager needle:
1.
In the nanoACQUITY UPLC Console, select Sample Manager from the
system tree.
2.
Click Control > Wash Needle.
Wash Needle dialog box:
3.
In the Strong Wash box, specify the volume for the strong wash solvent.
The default value is 0.0
µ
L. To omit strong wash solvent enter 0 in the
Strong Wash box, or leave it blank.
Summary of Contents for nanoACQUITY UPLC
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