4-4
Verifying System Operation
To prepare the sample:
1.
Pipette 1.0 mL (0.95 mL of 0.1% formic acid/water you mixed in the
previous section and 0.050 mL of 0.1% formic acid/acetonitrile mobile
phase) into the vial containing the peptides mixture.
2.
Cap the sample vial, and vortex-mix the sample for 10 seconds.
3.
Pipette 1.0 mL of 0.1% formic acid/water into an empty vial, and then
cap the vial. The mobile phase blank will be injected from this vial.
4.
Place the sample vials in the vial plate, noting the vial positions, and put
the plate in position 2 of the sample manager.
The peptides solution is stable for one week when stored at 8 ºC. You can
extend stability by freezing the sample at -20 ºC. However, for best results,
you should run the verification test immediately after preparing the sample.
Caution:
The sample degrades rapidly when contaminated with
endopeptidase or exopeptidase enzymes.
Preparing the system
Recommendation:
Flush the system with the appropriate solvents before
passing eluent into the column, optical detector, and/or mass spectrometer.
To prepare the system for verification:
1.
Connect and install the trap column.
2.
Install the analytical column in the heating and trapping module, and
then connect the column outlet to a suitable waste container.
3.
In the nanoACQUITY UPLC Console, perform these tasks if you have
not already done so:
a.
Prime the A1 and B1 solvent lines for 5 minutes.
b.
Prime the seal wash pump for one minute.
Warning:
Always observe safe laboratory practices when you use this
equipment and when you work with solvents and test solutions. Know
the chemical and physical properties of the solvents and test solutions
you use. See the Material Safety Data Sheet for each solvent and test
solution in use.
Summary of Contents for nanoACQUITY UPLC
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