Shutting down the system
2-25
3.
The heating and trapping module can operate overnight but should be
shut down over the weekend.
More than 72 hours (long-term)
To shut down the system long-term or indefinitely:
1.
Follow the preceding procedure for overnight or weekend shutdown.
2.
After flushing the column and letting it cool to ambient temperature,
disconnect the inlet and outlet tubes, and join them with a union.
Recommendation:
You should flush high-ionic buffers not only from the
column but also from the system. Flushing the column with
methanol/acetonitrile leaves that solvent in the fluid lines of the solvent
manager, sample manager, and detector, eliminating the risk of solute
precipitation.
3.
Pump water through the system for 10 to 20 minutes at 10
µ
L/min,
followed by isopropyl alcohol for another 10 to 20 minutes. Then turn the
pump off, leaving isopropyl alcohol in the fluid lines.
Caution:
If any system modules are to be used for another type of
analysis, ensure that the liquids pumped initially through the system
are miscible with methanol, water, methanol/acetonitrile, or isopropyl
alcohol. Likewise, before restarting the system, ensure that any residual
material not miscible with the initial methanol/water mobile phase has
been flushed thoroughly from the system with an appropriate
intermediate solvent.
Warning:
To prevent injury, set the power switch to off, and then
unplug its power cord from the AC outlet to completely interrupt power
to a system module.
The power switch on each system module controls the basic
operational state of that module. Nevertheless, a portion of the module
remains powered-on after the module is switched off.
Summary of Contents for nanoACQUITY UPLC
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