Preparing the detector
2-23
Conditioning the column
Conditioning the column involves running a solvent gradient through it
without injecting samples or running the Events table. The run time for
conditioning the column should equal the gradient table run time.
Tips:
•
Flush new columns with high organic (up to 85%) for 15 to 20 minutes at
your typical analytical flow rate.
•
To ensure best performance, flush with higher organic at the end of each
run.
Caution:
To prevent damage to the detector flow cell, ensure that the waste
solvent does not flow through the detector during this procedure. After
installing a new column, flush solvent through it
—
typically 10
column-volumes
—
and out to waste before connecting the column to the
detector.
To condition the column:
1.
Remove the column inlet line from the detector, and place the line’s end
in a small waste container.
2.
In MassLynx, open the Sample Set window, and select an inlet method
that includes the chromatographic conditions you want to use.
3.
In the Samples table, add an inlet prerun field.
a.
In the Run Samples page, select Samples > Format > Customize.
b.
In the Custom Field Display window, select Inlet prerun.
c.
To save the column as part of the window, save the sample set
format.
4.
Select method setup in the Sample Set window as a pre-inlet method,
and select a method for the inlet file (these methods can be the same).
5.
Run the sample set line. The system runs the condition column method
and then runs the separation method.
Summary of Contents for nanoACQUITY UPLC
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