Thermo Scientific EVOS M5000, Manual

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90 EVOS
™
M5000 Imaging System User Guide
Operation principles and technical overview
LED illumination The EVOS ™ M5000 Imaging System utilizes an adjustable intensity LED light
source provided by the proprietary, user-interchangeable LED light cube (see
below). Because the LED light source is as close as possible to the objective turret,
the number of optical elements in the channel is minimized. High-intensity
illumination over a short channel increases the efficiency of fluorophore excitation,
providing better detection of weak fluorescent signals.
In contrast to traditional fluorescence microscopy light sources that use mercury, a
toxic carcinogen requiring special handling and disposal, the LED light source of
the EVOS
™ M5000 Imaging System is more environmentally friendly, energy
efficient, and has a significantly longer life span (>50,000 hours versus 300 hours
for a typical mercury bulb and 1,500 hours for a metal halide bulb).
LED light cubes Each user-interchangable, auto-configured light cube contains an LED, collimating
optics, and filters. In addition to the channel dedicated to the transmitted light
from the condenser for brightfield contrast applications, the EVOS
™ M5000
Imaging System can accommodate up to four fluorescent or specialty light cubes
for multiple-fluorescence research applications.
The table below lists some of the common fluorescent and specialty light cubes
available from Thermo Fisher Scientific. For a complete list of available light cubes
and to inquire about custom light cubes, go to
thermofisher.com/evos or contact
Technical Support (see page 116).
Light cube Dye
DAPI DAPI, Hoechst ™ , BFP
TagBFP TagBFP
CFP ECFP, Lucifer Yellow, Evans Blue
GFP GFP, Alexa Fluor ™ 488, SYBR ™ Green, FITC
YFP EYFP, acridine DNA
RFP
RFP, Alexa Fluor ™ 546, Alexa Fluor ™ 555, Alexa Fluor ™ 568,
Cy ™ 3, MitoTracker ™ Orange, Rhodamine Red, DsRed
Texas Red
Texas Red
™
, Alexa Fluor
™
568, Alexa Fluor
™
594,
MitoTracker
™
Red, mCherry, Cy
™
3.5
Cy5 Cy ™ 5, Alexa Fluor ™ 647, Alexa Fluor ™ 660, DRAQ5 ™
Cy5.5 Cy ™ 5.5, Alexa Fluor ™ 660, Alexa Fluor ™ 680, Alexa Fluor ™ 700
Cy7 Cy ™ 7, IRDye 800CW
Specialty light cubes Dye
CFP-YFP em CFP/YFP (for FRET applications)
AO
Acridine RNA, simultaneous green/red with
FL color
AOred Acridine RNA, CTC formazan, Fura Red
™
(high Ca
2+
)
White Refracted light applications