20
EVOS
™
M5000 Imaging System User Guide
Focus on the
sample
1.
Use the
Coarse
and
Fine focus
sliders
to focus on the sample.
Alternatively, click
Coarse
and
Fine
autofocus
to autofocus on the sample.
2.
To limit the autofocus algorithm to a specific region along the Z-axis, use the
Autofocus Maximum
and
Autofocus Minimum
controls (blue triangles on
the Coarse focus slider) to set the upper and lower bounds.
Note:
You can also focus on the sample manually using the
focusing knobs
on the instrument (page 7).
Click
Enable focus knob
, then turn the focusing
knob until the region of interest is in focus.
3.
Repeat the focus procedure for each channel you want to capture.
4.
If you want to preserve the Z-Offsets between the channels,
select
Lock Z
.
•
When locked, focusing in one channel affects all channels.
•
When unlocked, focusing affects only the selected channel.
Note:
Z-Offset specifies the focus position in the selected channel relative to the
focus position in other channels. Setting the correct Z-Offset is especially important
when the fluorescent markers in different channels are in different focal planes.
Click next to Lock Z selection to view the recommended workflow for Z-Offset
set-up.
