18
EVOS
™
M5000 Imaging System User Guide
Capture images in a single channel
Select objective and
light source
1.
Place the vessel containing your sample on the stage using the appropriate
vessel holder.
Note:
When capturing images in fluorescence channels, place the light shield
box on the stage, over the sample. This is important for optimal image quality.
2.
Set the magnification using the objective selection wheel (page 7).
3.
Select the desired
Phase
option by turning the phase annuli selector (page 7).
Available options are:
•
Oly 4×:
Used for low magnification objectives (Olympus
™
4× PH)
•
4×/10×:
Used for medium magnification objectives (EVOS
™
4×/10× PH)
•
20×/40×:
Used for high magnification objectives (EVOS
™
20×/40× PH)
•
Brightfield
(phase contrast off)
The active objective and phase ring information is displayed above the
Channel buttons.
4.
Select a
Channel
to turn on the excitation light and enter the instrument in the
Live mode
. In this example, the DAPI channel has been selected.
In the Live mode, you can adjust the brightness and configure display settings
for the selected channel, and set the focus on the sample.
Note:
You can select only one light source at a time for Live mode to adjust
brightness and set focus. However, you can display and capture multiple
channels simultaneously.
