44
EVOS
™
M5000 Imaging System User Guide
8.
Select to autofocus at
Every interval
or
at
First interval of each run,
then click
Next
.
Note:
In a time lapse routine, images are captured at the end of each interval
of a run. For more information, see “Intervals”, page 46.
9.
If you have opted to capture
Z-Stack
images, define the
Top
,
In Focus
, and
Bottom
positions for the Z-Stack image set.
a.
If not already on, click the
Light
button to illuminate the sample as you
locate the boundaries and the In Focus position of the Z-Stack.
b.
To accept the
Current Z
as the
In Focus
position, click
Set
.
To change the
In Focus
position, move the
Coarse
and
Fine
focus
sliders
on the Capture tab to focus on the sample, then click
Set
.
c.
Move the focus sliders up from the Current Z position to the desired
Top
position (upper boundary of the Z-Stack), then click
Set
for Top.
d.
Move the focus sliders down from the Current Z position to the desired
Bottom
position (lower boundary of the Z-Stack), then click
Set
for Bottom.
Note:
Click the
Z number
button for the Top, In Focus, and Bottom positions
to jump to that position along the Z-axis.
