46
EVOS
™
M5000 Imaging System User Guide
13.
Enter the
Total Time
for Run 1 in
Hours
,
Minutes
, and
Seconds
.
You can create multiple Runs for a Time Lapse Routine, each with its own
duration and image capture frequency.
14.
Set the
Image capture options
for Run 1. Available options are
Frequency
,
Intervals
, and
As fast as possible
.
•
Frequency
: Enter the time in
Hours
,
Minutes
, and
Seconds
that must
elapse before a new set of images are captured.
For example, in an experiment with an image capture frequency of
2 minutes and 30 seconds, the images will be captured every 2 minutes
and 30 seconds after the initial set of images are acquired at time point 0.
•
Intervals
: Enter the total number of
time intervals
between the captured
image sets for a given run duration.
Images are collected at the end of an interval. For example, in a Run
experiment with a duration of 5 minutes and 2 intervals, the images will
be captured every 2 minutes and 30 seconds after the initial set of images
are acquired at time point 0.
•
As fast as possible
: Select this option to capture a new set of images
immediately after completing the previous set without any delay between
the sets.
The speed with which the images are captured depends on the specific
choices made for the routine such as the autofocus frequency and
exposure settings.
IMPORTANT!
To reduce phototoxicity, minimize the capture frequency and
light intensity, and maximize gain.
