EVOS
™
M5000 Imaging System User Guide
103
Calibrate oxygen
sensor
Calibrating the oxygen sensor ensures that the atmosphere in the environmental
chamber is replenished with the appropriate gasses in the correct proportion.
1.
Go to the
Settings
tab, then click
Incubator
to display the Incubator panel.
2.
Click
Oxygen Sensor Calibration
to open the Incubator Setup window.
3.
Verify that your gas connections have been correctly configured.
4.
Verify that the
Oxygen %
has been set correctly. If not, enter the correct value
for oxygen content.
5.
Select the
purge gas source
for your calibration (
Nitrogen
or
CO
2
).
6.
Click
Begin Calibration
. The EVOS
™
M5000 Imaging System automatically
calibrates the oxygen sensor for the proper functioning of the EVOS
™
Onstage
Incubator. The entire calibration process takes approximately three minutes.