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EVOS
™
M5000 Imaging System User Guide
6.
Capture time lapse images
Time Lapse tool
With the Time Lapse tool, you can set up your cells and program the EVOS
™
M5000
Imaging System to capture images (including Z-Stack images) at given intervals over a
time period based on your specifications. For repeat experiments, time lapse routines
can be saved, recalled, and edited.
Run a time lapse routine
Run a new time
lapse routine
1.
On the Capture tab, click
Automate
, then select
Time Lapse
to open the Time Lapse tool.
2.
Select the
Capture Channels
. You can select multiple channels in which to
capture time lapse images. In the following example,
DAPI
and
TX Red
channels have been selected.
3.
Select
Auto Focus
to run the autofocus algorithm during the
time lapse routine.
Unselect Auto Focus to capture time lapse images using the initial focus
positions set before you run the routine.
