2 • Description
Doc. 065330-05 03/2020
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2.6
Software Control
Use the AutoTrace software provided with the system to set operational
parameters and create method steps that automate Dionex AutoTrace 280
operation. You can create and store up to 24 methods in the AutoTrace software.
Through setup parameters, the system controls the flow rates of solvent and
sample in each solid-phase extraction step of the method. Up to five solvents can
be designated for the liquid sequences used in cartridge (or disk) preconditioning,
sample loading, cartridge (or disk) rinsing, and elution.
The remainder of this section describes the key steps (both standard and optional)
in an SPE method. For more information, refer to the AutoTrace software Help.
2.6.1
Standard Operations
The table below describes the standard operation steps in an SPE method.
Operation
Description
Conditioning the
cartridge or disk
Use
Condition Cartridge
or
Condition Disk
to pass
the solvent through the cartridge or disk, respectively,
and direct the effluent to waste.
Loading the sample
Use
Load Sample
to pump sample from the sample
container to the cartridge or disk for extraction.
Rinsing the cartridge
or disk
Use
Cartridge Rinse
or
Disk Rinse
after loading the
sample path and the cartridge or disk, respectively.
The effluent is directed to waste.
Washing the syringe
solvent
Use
Wash Syringe
to prewash the liquid handling
syringe with the selected solvent. The resulting
solvent waste exits through the waste port.
Eluting the sample
Use
Elute to Collect
to pass solvent through the
cartridge or disk and direct the eluent into the
collection container.
