4. Optical System
34
Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0
2011-09
4.1.2
Fluorescence Top/Bottom Optics
Flash light
enters the optical system and is focused by the condenser onto the
entrance slit of the excitation monochromator. The wavelength and bandwidth of
the excitation light is selected within the monochromator. After passing the
monochromator, the excitation light is coupled into a fiber bundle which guides
the light to the top or bottom measuring head. The light is then focused into the
sample by the top/bottom lens system (Figure 13, left-hand side).
The fluorescence emission light is collected by the top/bottom lens system again,
coupled into the fluorescence fibers bundle (Figure 13, right-hand side) and
guided to the detection system.
Z-Positioning (Top Fluorescence only)
The
Z-position
of the fluorescence top optics can be adjusted. As light is
refracted onto the sample liquid surface, a Z-adjustment helps to maximize signal
to noise.
The fluorescence measuring Top and Bottom Optics are built from the following
components:
1. Fluorescence Intensity Lens System Top/Bottom
2. Fluorescence Fiber Bundle
Fluorescence Intensity Lens System
The exit side of the bundle acts as a color specific light source. The lens system
at the end of the excitation top and bottom fibers is designed to focus the
excitation light into the sample and also collect the fluorescence light and focus it
back onto the fluorescence fiber bundle.
The objective lenses are made from fused silica. This material provides high UV
transmission and is virtually void of auto-fluorescence.
Fluorescence Fiber Bundle
The fiber bundle plugged into the top/bottom measuring head contains a
homogeneous mixture of both excitation and emission fibers. The emission fibers
guide the fluorescence light to the emission monochromator head where a lens
system focus the light onto the entrance slit of the emission monochromator.
