7. Quality Control
100
Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0
2011-09
7.4.1.3 Detection Limit Top Fluorescein 1536-well Plate
Pipette 10 µl of a 1 nM Fluorescein solution (diluted in 0.01 M NaOH) and 10 µl of
the blank solution (0.01 M NaOH) into the wells of a Greiner 1536-well plate
(black, flat bottom) according to the plate layout.
Material:
Greiner 1536-well plate, flat bottom, black
10 µl p tips
1 nM Fluorescein (diluted in 0.01 M NaOH)
0.01 M NaOH
Plate layout:
<>
1
…
5
…
9
…
13
…
17
…
21
…
25
…
29
…
33
…
37
…
41
…
45
…
A
F B F B F B
F
B
F
B
F B
…
E
F B F B F B
F
B
F
B
F B
…
I
F B F B F B
F
B
F
B
F B
…
M
F B F B F B
F
B
F
B
F B
…
Q
F B F B F B
F
B
F
B
F B
…
U
F B F B F B
F
B
F
B
F B
…
Y
F B F B F B
F
B
F
B
F B
…
AC
F B F B F B
F
B
F
B
F B
...
(F….10 µl 1 nM Fluorescein; B…..10 µl 0.01 M NaOH = Blank)
Measurement Parameters:
Measurement mode:
Fluorescence Top
Excitation wavelength: 483 nm
Emission wavelength: 525 nm
Excitation bandwidth:
20 nm
Emission bandwidth:
20 nm
Gain:
Optimal
Read Mode:
Mode 2 (100 Hz)
Number of flashes:
12
Lag time:
0 µs
Integration time:
40 µs
Plate definition file:
GRE1536fb.pdfx, whole plate
Z-Position:
Calculate from A1
