4. Optical System
2011-09
Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0
39
Z-Positioning
The Z-position of the polarization optics can be adjusted (Figure 14). As light is
refracted onto the sample liquid surface, a Z-adjustment helps to maximize the
signal to noise ratio.
The Fluorescence Polarization Optics (Figure 16) consist of the following
components:
•
Polarizer
•
Rotator
•
EX Filter 1 – 4
•
Dichroic mirror 1 – 4
•
Analyzer
Figure 16: Fluorescence Polarization Optics.
Polarizer
A polarizer is a device for producing plane-polarized light.
Rotator
An LC (Liquid Crystal) rotator changes the plane of the polarized excitation light.
EX-Filter
In many cases, fluorescence emission spectra do not depend on the exact
excitation wavelength; therefore, for a maximum total fluorescence signal, broad
excitation band pass filters (10 – 40 nm) should be used. For each LED an
appropriate EX-filter is installed.
