2. General Description
2011-09
Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0
19
The Chroma-Glo luciferase assay generates red and green (dual-color)
luminescence from two luciferases within a single well and upon a single reagent
addition. This homogeneous dual-reporter gene assay permits each reporter to
be measured independently by detecting one well at two different wavelengths
(red and green).
Luminescence Scan
The
INFINITE M1000 PRO
is capable of recording emission spectra of
luminescent signals. Luminescence substrates providing stable light output are
required for luminescence scans.
As an example, emission spectra of different luciferase types (new recombinants
of Renilla or Firefly luciferase) can be recorded in order to define emission
maxima. Also environmental influences on the spectral behavior of luciferases
can be studied (pH-value, solvent, buffer).
The luminescence scanning procedure is operated by the fluorescence emission
optics, therefore additional information on the luminescence scan can be found
in chapter 4.1 Fluorescence Intensity System and chapter 5.3 Optimize
Fluorescence Measurements.
2.2.4 AlphaScreen/AlphaLISA
Caution
The AlphaScreen/AlphaLISA module uses a high-power laser light source. Do
not stare into the instrument while a measurement is running.
The
INFINITE M1000 PRO
is able to measure Amplified Luminescent Proximity
Homogeneous Assays (AlphaScreen and AlphaLISA). Due to their
nonradioactive, homogeneous and sensitive nature, these bead-based
technologies are perfectly suited for the study of biomolecular interactions.
Upon illumination with a high-energy light source, the photosensitive molecules
contained in the donor beads produce high levels of oxyradicals. These
oxyradicals are able to travel to the acceptor beads and trigger a cascade of
reactions that ultimately lead to the generation of a strong chemiluminescent
signal.
Note
AlphaScreen/AlphaLISA measurements are only possible as endpoint
measurements in white or light gray microplates and cannot be
performed in combination with the injector system and the heating
system.
