7. Quality Control
110
Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0
2011-09
7.4.4 Luminescence
7.4.4.1 Crosstalk ATP 96-well Plate:
Pipette the reagents into the wells of a Greiner 96-well plate (white, flat bottom)
according to the Plate Layout.
Material:
ATP Kit SL 144-041, BioThema AB
Greiner 96 well plate, flat bottom, white
200 µl p tips
Plate layout:
Pipette 200 µl of the Blank into the wells A1 – D1 and A3 – D10.
Pipette 40 µl of ATP standard 10
-7
M into the wells A2 – D2, add 160 µl of ATP
reagent and mix in well (
use fresh tip for each well); ATP reagent must NOT
be contaminated with ATP standard!
<>
1
2
3
4
5
6
7
8
9
10
11
12
A
Bx
ATP
Bx B B B B B B B
B
Bx
ATP
Bx B B B B B B B
C
Bx
ATP
Bx B B B B B B B
D
Bx
ATP
Bx B B B B B B B
E
F
G
H
ATP….200 µl 2*10
-8
M ATP; B….200 µl Blank, Bx…200µl Blank used for
crosstalk check
Measurement Parameters:
Before pipetting the plate, prepare instrument for measurement:
Parameters:
Measurement mode:
Luminescence
Integration time:
1000 ms
Plate definition file:
GRE96fw.pdfx
Part of the plate:
A1 – D10
Start measurement immediately after pipetting!
Evaluation:
Calculate the crosstalk for each well separately. The following wells are charged
together: A2 with A1, A2 with A3, B2 with B1, B2 with B3, C2 with C1, C2 with
C3, D2 with D1, and D2 with D3. The average of these 8 values must be within
the specified limits, see chapter 7.3 Acceptance Criteria (under Luminescence
Crosstalk).
)
(
100
*
)
(
%
B
ATP
B
B
CT
wellx
X
−
−
=
B
X
Blank wells A1 – D1 and A3 – D3, respectively
ATP
wellx
ATP wells A2 – D2, respectively
B
Average of wells A4- D10 (Blank)
