7.
Quality
Control
2011-09
Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0
103
Evaluation:
Calculate the detection limit in pM:
B
B
F
stdev
*
3
*
)
mean
(mean
1000
imit
DetectionL
−
=
mean
F
average of wells filled with 1 nM Fluorescein
mean
B
average of wells filled with Blank
stdev
F
standard deviation of wells with Blank
7.4.1.6 Uniformity Bottom Fluorescein 384-well Plate:
Pipette 100 µl of the 1 nM Fluorescein solution into the wells of a Greiner 384-
well plate (black; µClear, flat bottom) as described in chapter 7.4.1.5 Detection
Limit Bottom Fluorescein 384-well Plate.
Material:
See chapter 7.4.1.5 Detection Limit Bottom Fluorescein 384-well Plate (except
Blank = 0.01 M NaOH).
Measurement Parameters:
See chapter 7.4.1.5 Detection Limit Bottom Fluorescein 384-well Plate
Evaluation.
Calculate the uniformity:
F
F
mean
100
*
stdev
(%)
Uniformity
=
mean
F
average of wells filled with 1 nM Fluorescein
stdev
F
standard deviation of wells 1 nM Fluorescein