5. Operating the INFINITE M1000 PRO
2011-09
Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0
57
Intensities:
Parallel and perpendicular intensities are calculated using the following formulas:
par
par
RFU
G
I
Δ
=
*
cross
cross
RFU
I
Δ
=
Polarization:
cross
par
cross
par
I
I
I
I
P
+
−
=
Anisotropy:
cross
par
cross
par
I
I
I
I
A
*
2
+
−
=
Total Intensity:
cross
par
I
I
I
total
*
2
+
=
5.3.3 Instrument
Parameters
Gain Settings
The
INFINITE M1000 PRO
fluorescence detection system uses analog to digital
(A/D) conversion of the PMT signal. The gain setting controls the amplification of
the PMT when converting fluorescence light into electrical current. The A/D
converter needs a suitable input range of PMT current to provide the proper
signal to noise ratio (S/N) on the one hand, and linearity on the other hand.
Therefore, the gain should be optimized based on the wells with the highest
concentration give the highest possible readings. For best possible signal
resolution it is recommended to use the “Optimal Gain” setting.
Note
If any well of interest is assigned “OVER” (overflow), you may manually
reduce the gain, or select an automatic gain option (see the software
manual).
