Rotor-Gene Q MDx CE User Manual 02/2022
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Cooling
Illustration of the heating and cooling system.
3.1.2
Optical system
With a choice of up to 6 excitation sources and 6 detection filters combined with a short, fixed
optical path, the Rotor Gene Q MDx can be used for multiplex reactions, ensuring minimum
fluorescence variability between samples and eliminating the need for calibration or compensation.
Samples are excited from the bottom of the chamber by a light-emitting diode. Energy is transmitted
through the thin walls at the base of the tube. Emitted fluorescence passes through emission filters
on the side of the chamber and is then collected by a photomultiplier. The fixed optical path ensures
consistent excitation for every sample, which means that there is no need to use a passive internal
reference dye such as ROX™.
