Rotor-Gene Q MDx CE User Manual 02/2022
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By default, all samples are shown in the display. Samples can be removed from or added to the
display using the toggler to the right. The toggler consists of colored cells, each of which
corresponds to a sample in the display. Samples with a brightly colored cell are displayed, while
samples with a faded cell are not displayed. Samples can be switched on or off by clicking on the
cell or by dragging the mouse pointer across several cells at time.
We recommend performing Manual Gain Adjustment as follows.
1.
Adjust the temperature in the
Manual Gain Adjustment
window to the acquisition temperature
required for the run.
Note
: The temperature will not be adjusted while the Rotor-Gene Q MDx is operating. Restart
the Rotor-Gene Q MDx to apply changes made to the temperature.
2.
Click on
Start
. The run begins. The Rotor-Gene Q MDx temperature is adjusted to the
temperature specified in the window. The graphs in the window start to display data.
3.
Wait for the temperature to stabilize.
4.
Note the end point fluorescence (Fl) reading.
5.
If the Fl reading is not at the required level, click on
Edit Gains…
and edit as required. This
process may not be instantaneous, as the Rotor-Gene Q MDx takes approximately 4 s to
acquire each point in each channel, and during this time the user interface is deactivated.
6.
Repeat the process until the FI is at the desired level.
7.
Click on
Stop
. If the run is still acquiring data when the
Stop
button is clicked, the Rotor-Gene
Q MDx finishes acquiring first, and then stops. This process can take up to 5 s for each
acquiring channel.
New Run Wizard window 4
This window summarizes the run. Check the parameters and if they are correct, click
Start Run
. You
will be prompted for a file name. You can also save the run settings as a template for future runs
using the
Save Template
button.
