Rotor-Gene Q MDx CE User Manual 02/2022
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Run setup
To set up the run, select
Nucleic Acid Concentration Measurement
from the Quick Start wizard.
Note
: Ensure that a positive control, such as a high concentration standard, is run in tube position
1. Without a positive control, the software will be unable to optimize gain settings for maximum
sensitivity. You will be prompted for this before each run.
Analysis
Concentration analysis operates by relating the fluorescence level to a concentration value. Two
analysis models are available. The optimal analysis to choose depends on the chemistry and
application.
“Linear Regression” analyzes data by assuming a linear relationship and estimating unknown
values on the basis of a generated linear model. It determines measurement error by examining the
deviation of the readings from a linear model. If concentration readings are linear, this is the most
suitable analysis because it provides statistical analysis of variation (ANOVA) to the user.
