Rotor-Gene Q MDx CE User Manual 02/2022
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3.
The “Calibration Settings” information is usually correct. To modify it, if necessary, click
Edit
.
The
Calibration Settings
window appears.
4.
Ensure that:
o
The tube indicated in
Tube Position
contains a PCR product that will show a melt peak on
the green channel.
o
The final ramp temperature will not burn the sample, yet will be high enough to allow it to
melt.
o
The hold time is sufficient to denature the sample.
o
The denature offset is set appropriately. The default of 0
o
C is appropriate for most melts.
Melts with very sharp transitions may require a denature offset of –0.5
o
C to –2
o
C, as
determined by the user, to ensure that melt transition is detected.
You can also define a Denature step by introducing a new Hold step. Click on
Insert before
and
select
New Hold at Temperature
from the menu. The calibration settings will appear.
