Rotor-Gene Q MDx CE User Manual 02/2022
104
Flip sign of dF/dT
:
Before defining peaks, ensure that the dF/dT sign is correct for the data set to
give positive peaks.
Defining peaks:
In melt curve analysis, peaks can be defined and reported using different
methods. One is to automatically call all the peaks for each sample. The other
is to assign peaks to bins, which is useful for genotyping.
Bins define the area where peaks are expected to occur. The melt curve
analysis software clusters peaks into bin groups, based on actual peak values
in the curve. Bins can be edited if required.
Any peak that is within the defined range of the bin will be assigned to the
bin. If there are 2 bins close together then the peak will be assigned to the
closest bin.
Note
: The bins should not be visually positioned to estimate peak positions. Set
the bins in the approximate area of interest, then use the actual reported values
in the results table for a more accurate result.
Peak Bins
:
To define a bin, click the
New Bin
button, then click and hold on the graph to
define the center of the bin. To add another bin, repeat the process. Use the
Remove
button to delete bins.
Threshold
:
To set the threshold (y axis), click the
icon, then click and hold on the
graph and drag the threshold line to the desired level.
Temperature Threshold
:
To set a temperature threshold (x axis), click the
icon, then click and hold
on the graph and drag the threshold line to the right. This eliminates the
threshold line for the lower temperatures.
Note
: This is useful when there is noise in the signal at low temperatures.
Reports
This opens the
Report Browser
where a report can be chosen to preview. A report can be generated
based upon the currently selected channel, or a multichannel genotyping report can be generated.
