Rotor-Gene Q MDx CE User Manual 02/2022
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10.6
Sample preparation
Sample degradation should be avoided during purification and storage. Avoid excessive amounts
of inhibitors, such as from ethanol carryover. To improve HRM results, we recommended keeping
the amount of template used consistent between samples. Spectrophotometric analysis for
determining DNA concentration and purity is recommended. We recommend QIAGEN kits for
sample preparation.
Note
: At 260 nm one absorbance unit is equal to 50 µg/ml DNA. Pure DNA will provide a 260
nm to 280 nm ratio of 1.8.
10.7
Software setup
1.
Open a new run file by selecting
New…
from the
File
menu. In the Advanced wizard, select
HRM
.
