Rotor-Gene Q MDx CE User Manual 02/2022
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General Description
The Rotor-Gene Q MDx is an innovative instrument that enables high-precision real-time PCR, and
that is highly suited for in vitro diagnostic applications in combination with QIAGEN IVD marked
kits.
The powerful and user-friendly software provides simplicity for beginners as well as an open
experimental platform for advanced users.
3.1
Rotor-Gene Q MDx principle
3.1.1
Thermal performance
The Rotor-Gene Q MDx uses a sophisticated heating and cooling design to achieve optimal reaction
conditions. The unique rotary format ensures optimal thermal and optical uniformity between
samples which is critical for precise and reliable analysis.
Samples spin continually at 400 rpm during a run. Centrifugation prevents condensation and
removes air bubbles, but does not pellet DNA. In addition, samples do not need to be spun down
prior to a run.
Samples are heated and cooled in a low-mass–air oven. Heating is achieved by a nickel-chrome
element in the lid. The chamber is cooled by venting the air out through the top of the chamber
while ambient air is blown up through the base.
Heating
