PerkinElmer Caliper IVIS Spectrum, Hardware Manual

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35
IVIS
®
Spectrum Hardware Manual
7
Operating the IVIS
®
Spectrum
Restarting the System . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Gas Plumbing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Door Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Centering a Subject in the Field of View . . . . . . . . . . . . . . . . 39
Fluorescent Imaging . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Imaging Basics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
System Shut Down Procedure . . . . . . . . . . . . . . . . . . . . . . 42
The IVIS Spectrum is intended for use in biophotonic or fluorescent imaging
procedures. The system is designed to detect extremely low-level light
emissions that are orders of magnitude dimmer than can be detected by the
naked eye. The IVIS Spectrum allows you to monitor and record cellular and
genetic activity within a living organism, in real time. The imaging system
captures, quantifies, and images the light emitted by a sample.
7.1 Restarting the System
NOTICE
All components of the IVIS Spectrum should be left on unless the system will
not be used for more than 30 days. It is also important to leave the system on
to enable automatic overnight electronic background measurements.
Periodically rebooting the computer is permissible and does not affect the
camera operation.
Electrical Control & Status
Lights
The IVIS Spectrum console has two electrical switches and one status light
(Figure 7.1)
. The color of the status light indicates the state of the instrument
(Table 7.1)
. The main power on/off switch (circuit breaker) is located on the rear
panel and controls the AC power to the instrument.
The front panel contains a recessed toggle switch that distributes power within
the system console. This switch can be in the standby position (down) or on
position (up) and is equipped with a locking mechanism to prevent accidental
activation. The shaft of the switch must be pulled out to allow movement up or
down. The gas control valve is to the left of the toggle switch. For more details
on the gas control valve, see Gas Plumbing , page 37 .
CAUTION
!
CAUTION! Placing the toggle switch in standby mode turns off the
ThermoCube cooling unit and camera power supply. All low voltage
equipment remains on.