PerkinElmer Caliper IVIS Spectrum, Hardware Manual

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7. Operating the IVIS
®
Spectrum
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Selecting an Excitation
Filter
In fluorescence mode, the excitation filter is automatically selected based on the
wavelength of the emission (imaging) filter selected from the IVIS System
Control panel. However, you can override the automatic selection in the IVIS
System Control panel.
The excitation filters are housed in a filter wheel module that is not accessible
to the user. Unlike the emission filters, the user cannot replace these filters.
Contact IVIS Imaging Systems Technology Support if an excitation filter must
be replaced.
7.6 Imaging Basics
Black Paper
Although the platform is black anodized, it is recommended that you image
items on a high quality black paper, especially biological specimens. Caliper
Corporation has surveyed many types of paper and recommends Swarthmore,
Artagain, Black, part no. 445-109, size 8.5 inch x 11 inch. This paper prevents
illumination reflections and helps keep the stage clean.
Low Fluorescence Mat
When operating the system in the fluorescence mode, use the Low Fluorescence
Imaging Mat (part no. 117839) to reduce background fluorescence. The mat is
made from a low reflectance textured plastic that is easily cleaned.
Glowing Materials
Always keep in mind that nearly EVERYTHING glows (that is, has the
potential to phosphoresce and contaminate the image). Most plastics, almost all
tape, plants, paint, rodent food (mostly plants), mouse urine, and animal
bedding have been found to glow.
Use caution when introducing materials into the IVIS
®
Spectrum. It is advisable
to prescreen all items by imaging them alone, before imaging them with
samples under study. Caliper recommends using non-powdered gloves when
working with IVIS Spectrum equipment.
7.7 System Shut Down Procedure
Caliper Corporation does not recommend power cycling the IVIS Spectrum
(turning the system components on and off) on a daily basis. Leaving the system
on keeps the camera cold and the electronics systems stable. The software is
preconfigured to run automatic background accumulation and self-diagnostics
overnight. The platform temperature and the computer monitor may be turned
off.
If it is necessary to shut down the system for any reason, such as long term
storage (more than 30 days), it is important to follow the procedure below.
1. Close the Living Image
®
software and save any information of interest at the
prompt.
2. Turn off the computer.
3. Turn off any gas to the imaging chamber and disconnect the gas supply if
necessary. If you are using the XGI-8 Gas Anesthesia system, follow the
shut down procedure in the XGI-8 Anesthesia System User Manual .
4. Turn off (standby) the toggle switch on the front of the IVIS Spectrum.