PerkinElmer Caliper IVIS Spectrum, Hardware Manual

Page: 52 / 74

8. Troubleshooting
46
8.2 Photographic Image Is Unacceptable
Default camera controls are programmed during the initialization of the Living
Image
®
software. Changes to these settings can greatly affect the photographic
image. Check that the exposure time, f/stop and binning levels are set to the
default conditions.
If the f/stop is open too wide, the image may be saturated and less focused.
Binning to a higher value or increasing the exposure time can produce an image
that is too bright and lacks resolution. If you have questions about the
photographic settings, please contact IVIS Imaging Systems Technology
Support.
Also, refer to the
Living Image Software Manual for further details on acquiring
images.
Problem Possible Cause Corrective Action
Image is streaked. Subject moved during the
exposure.
Check to see if the subject may have moved. If the
subject is not on the sample stage, it is probably on the
floor of the imaging chamber. If the sample has moved,
locate and reanesthetize it. If gas anesthesia is being
used, confirm that the anesthesia is turned on and the
flow rate is appropriate.
Image is blurry. Subject height is not correctly
entered in the Living Image
software.
If you are using the “Sample Height”setting to determine
the focus, confirm that the correct height of the sample
is entered. Alternatively, Auto-Focus may be used to
determine the sample height.
If you are using the “Manual”setting to determine the
focus, confirm that the focus has been manually
adjusted.
Incorrect f/stop setting. The f/stop for photographs should be set to f/8. An f/stop
smaller than 8 reduces the depth of field in the
photograph.
A white spot appears in the
center of the field of view.
An excessively moist
environment in the imaging
chamber can result in
condensation on the CCD
window
(Figure 8.2)
.
Remove excess moisture in the imaging chamber and
allow it to thoroughly dry. Place a desiccant in the imaging
chamber to decrease the drying time.
Do not touch or let specimens touch the window at the
top of the imaging chamber. For more details on the care
of this window, see page page 52 .
If the problem persists, contact IVIS Imaging Systems
Technology Support for assistance.
Figure 8.2 Condensation example