PerkinElmer Caliper IVIS Spectrum, Hardware Manual

Page: 58 / 74

9. Care & Maintenance
52
NOTICE
Caliper makes no claims as to the sterility of the IVIS imaging chamber after
using the solutions in Table 9.1. Please refer to the manufacturer’s literature
for information as to the applicability of the compound for the organism of
interest. Consider dedicating an imaging system for immunodeficient animals.
This will reduce the risk of cross contamination.
Cleaning the Imaging
Platform
It is recommended that you use a lint-free wipe, such as Scott Pure
®
wipe or a
Kaydry EX-L
®
wipe to minimize the presence of particulate matter in the
imaging chamber.
After saturating a lint-free wipe, clean the internal surfaces using a gentle
circular motion. Do not pour or spray the solution directly onto internal
surfaces. Rinse surfaces using a wipe saturated with sterile deionized water. Do
not allow puddles of water to remain on the surfaces.
Avoid cleaning the ceiling of the image chamber except when necessary. When
cleaning the chamber ceiling, use extreme care to avoid damaging the lens
protection window and the light diffusers in this area.
To avoid any phosphorescence from the cleaner, be sure that the surfaces are
dry before using the imaging chamber.
9.2 Cleaning the Lens Protection Window
Contact IVIS Imaging Systems Technology Support for information about
cleaning or sterilizing any of the optical components or replacing the optical
filters. A clear window at the top of the imaging chamber protects the lenses and
other optical components from contamination. Do not touch or allow subjects
to touch this window, otherwise image quality may be impaired. Further, the
glass window could be cracked or broken if struck by a hard object. The lens
protection window should be cleaned periodically.
To clean the surface of the lens protection window:
1. Blow off any accumulated dust using a Dust-Off
®
compressed gas duster.
2. Dampen a lint-free cloth with isopropyl alcohol and gently swab the
window surface to remove any smudges or streaks.
3. Repeat step 2 using a clean area of the cloth or a new cloth until the window
is clean.
Table 9.1 Acceptable cleaning solutions for the IVIS
®
Spectrum imaging chamber
Cleaning Solution Manufacturer
Cidexplus ® Solution (3.4% glutaraldehyde) Johnson & Johnson Medical
70% methyl alcohol/30% deionized
water solution
70% ethyl alcohol/30% deionized water
solution
Sporicidin
®
Sterilizing Solution (1.56%
phenol)
Sporicidin International
Clidox-s
®
Disinfectant Pharmacal Research Laboratories, Inc.