PerkinElmer Caliper IVIS Spectrum, Hardware Manual

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IVIS
®
Spectrum Hardware Manual
7.5 Fluorescent Imaging
The IVIS
®
Spectrum has both bioluminescent and fluorescent imaging
capabilities. To change from bioluminescent to fluorescent operation, select
fluorescence mode in the IVIS System Control panel and select an appropriate
excitation and emission filter
(Figure 7.6)
.
A 150 Watt quartz tungsten halogen lamp with a dichroic reflector provides the
fluorescence excitation light. The Living Image
®
software controls the lamp
intensity level. The fluorescence light source is a module located behind the rear
panel and is not user serviceable.
The lamp output is delivered to the excitation filter wheel assembly located in
the rear compartment, where it is collimated and transmitted through a
fluorescence excitation filter. The 12 excitation filter wheel locations provide
10 fluorescence filters. Two filter slots contain a light block that is used during
bioluminescent imaging to prevent external light from entering the imaging
chamber. The 12-position excitation filter wheel is motorized and controlled
through the Living Image software.
Upon leaving the excitation filter, the light enters an optical switch that directs
the light to either the top of the imaging chamber or to the bottom of the stage.
When top illumination is selected, the light is equally split to four diffusers to
provide uniform stage illumination. When bottom or trans-illumination is
selected, the excitation light is directed to one of the 2 mm diameter holes in the
aperture plate located in the stage surface. This removable plate has a 15 x 23
array of 2mm diameter holes which provide discrete illumination locations that
are user selectable.
You can replace the array plate with a 3 mm thick glass plate or a well plate
holder to provide alternate illumination possibilities. A blank plate is also
provided for when the bottom illumination equipment is not in use. For more
details on how to use the top and bottom illumination systems, see the Living
Image Software User’s Manual .
Selecting an Emission
Filter
The IVIS Spectrum has a factory-installed filter set. The filters are mounted on
a rotating filter wheel and can be removed or replaced with filters with other
spectral characteristics. For more details, see Emission & Excitation Filter
Wheels , page 29 . For information on how to replace a filter, see page 53 . See
the Living Image
®
Software Manual for more details on how to select an
emission filter.
Figure 7.6 IVIS System Control panel
Choose
luminescent or
fluorescent
imaging