Perkin-Elmer RamanStation 400 Series, Getting Started Manual

Page: 79 / 98

Using the RamanStation 400 . 79
SOP-7: Sample Positioning when using a Fiber Optic
Probe
Monitor allows you to collect spectral data in the Live display while optimizing the probe
position, laser power and exposure time to enhance the signal-to-noise ratio of spectral data.
When measuring samples inside a container (for example, a bottle) it can be used to ensure
that you are focused on the material of interest and not the container.
The data that is displayed on the Live display when monitoring cannot be saved.
NOTE: To see the Live display, the
Show live display
option must be selected on the Setup
Instrument Data Collection tab.
1. Ensure that the RamanStation 400F is switched on.
If not, follow SOP-1.
2. Select
Monitor
from the measurement menu.
OR
Click on the Measurement toolbar.
The Live tab will be displayed and real-time spectral acquisition will commence.
3. Adjust the
Laser power (%)
on the Live display to an appropriate value for your
sample.
4. Adjust the Exposure time (s).
This will automatically acquire another background.
The sample may now be positioned and aligned correctly for optimized data collection
by adjusting the distance between the Fiber Optic Probe and the sample.
5. If required, adjust the Laser power, Exposure time and probe position further to
optimize the conditions for your sample.
6. When you have optimized your experimental parameters, click to exit the Live
display.
You can now collect and save spectral data. To set up an experiment and collect data using
the FOP please refer to SOP-4.
NOTE: If you have
Show Live Display
selected on the Setup Instrument Data Collection tab
and you select
Preview
on the Sample Table, then you can preview each sample in
the Live display after pressing
Scan
. When you have adjusted the settings, click Scan
again to collect data.