Perkin-Elmer RamanStation 400 Series, Getting Started Manual

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74 . RamanStation 400 Series Getting Started Guide
10. Load the sample bottle into the versatile sample holder.
11. Close the sample compartment lid.
The instrument can now be used to position the sample (SOP-5), and set up the
experimental parameters and collect data (SOP-4).
SOP-4: Setting-Up Experimental Parameters and
Collecting Data
This SOP is designed to act as a quick guide to help you start collecting data. The exact
setup for optimized spectral collection is sample dependent, and we recommend that you
modify these settings to suit your needs.
NOTE: If you are using a Triggered Fiber Optic Probe, refer to the Raman Triggered Fiber
Optic Probe leaflet (L1321940) supplied with the probe for configuration and usage
details.
1. Ensure the RamanStation 400 is switched on.
If not, follow SOP-1.
2. If you did not choose to connect to your instrument on starting Spectrum software,
select
Instruments
from the Setup menu and then
Select Instrument
from the sub-
menu.
3. Select your Raman instrument and then click
Connect
.
4. Select
Raman Instrument
from the Setup menu.
The Setup Instrument tabs are displayed in the dialog pane.
5. If required, select the appropriate
Accessory
from the drop-down list on the Setup
Instrument Basic tab.
The accessories available to you will depend on your instrument configuration.
Depending on the Accessory you have selected, further options may become available.
See
SOP-3: Installing and Changing Sampling Holders
and
SOP-5: Selecting the Fiber
Optic Probe
for further information.
6. Using the Instrument Settings toolbar, check and set the instrument parameters, such
as the
Start
and
End
points of the scan range (in Raman Shift, for example, 3200 cm
-1
to 100 cm
-1
), and enter a unique
Sample ID
and
Description
.
7. Set the
Exposure Time
and number of
Accumulations
.
You can define the Accumulations as a number of Exposures, or as a number of minutes
or seconds.
Typical values for Exposure Time and Accumulations are 5 seconds and 5 exposures,
respectively. Spectra may be optimized by increasing or decreasing the Exposure Time
and number of exposures. To obtain a good signal-to-noise ratio, you should co-add a
number of spectra by choosing a suitable number of exposures. A minimum of two
exposures is recommended for cosmic ray removal.