Perkin-Elmer RamanStation 400 Series, Getting Started Manual

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Using the RamanStation 400 . 83
SOP-10: Wavelength Calibration
Wavelength calibration should only be performed if the instrument has failed a wavelength
calibration verification.
1. Ensure the RamanStation 400 is switched on.
If not, follow SOP-1.
2. Attach the external neon calibration accessory (L1320220) to the rear panel of the
RamanStation 400 Series.
Push the large connector into the socket labeled CAL ASSY and carefully connect the
small cable to the COL coupler, ensuring that the cable is fully inserted.
NOTE: As wavelength calibration is not required on a regular basis, the external neon
calibration accessory is not included in the basic RamanStation 400 Series package.
The external neon calibration accessory can be purchased separately.
3. Ensure that the RamanStation 400 Series sample compartment is closed and, if you
have a RamanStation 400F, ensure that any external accessory is capped or covered, to
prevent stray light interfering with the measurement.
If you have a RamanMicro 300 Accessory connected to your RamanStation 400F, ensure
that the microscope illumination is switched off.
4. Select the Setup Instrument Advanced tab and click
Toolbox
.
The Adjustments Toolbox dialog is displayed.
5. Select .
The Wavelength Calibration dialog is displayed.
6. Ensure that you have followed the instructions on the dialog, and then click
Next
.
The instrument will now perform the wavelength calibration routine. This will take some
time. When the calibration is complete, the result is displayed.
7. Disconnect the neon calibration accessory and refit the screw-in cap to the COL port.
The instrument is now wavelength calibrated.