Particulate Systems NanoPlus, Operator'S Manual, Page: 48 / 252

Group Parameter Description
Size
Measurement Accumulation

Times
Enter the number of integration cycles for the
measurement; the default is 70. At this value, the
software determines 70 ACFs and calculates 70
realtime diameters from each ACF, ultimately
calculating one averaged ACF from 70 ACFs to give
the mean diameter.
Increase the number of cycles if the intensity of the
sample is weak or if the signal is too noisy to obtain a
stable autocorrelation function. If the intensity is less
than 10,000 cps, use the following formula as a
guideline to input the number of cycles:
Number of integration cycles = 10,000 (cps) /
intensity of the sample (cps) x 10
Size
Measurement
(Details)
This group of
parameters
displays when
Linear
correlator is
selected.
Correlation Channel Select the correlation channel of the correlation
calculation. The correlation channel represents the
number of bins into which the size data are
distributed. It is the size resolution (the more
channels, the more divisions on the size scale, i.e., X-
axis).
Select Auto if you are not sure which channel to use.
Correlation Method Select TD (Time Domain), TI (Time Interval), or
Auto . Typically, TD is for large particles having
stronger scattering levels, and TI for small particles
of less than 10 nm having weaker scattering levels.
Select Auto if you are not sure which to choose.
Sampling Time (

s)
Select the sampling time from the list. The sampling
time is the time after which the signal is collected
from the sample by the instrument. Appropriate
sampling time must be selected to get a smoother
ACF and for accurate size measurement.
Select Auto if you aren’t sure which time to use.
Titrator Titration Mode This parameter determines the mode for pH titration
vs. size, diluting/priming, or circulation of the
sample.
Choose pH titration , Pipetting , or Circulation .
The Titration Mode is applicable only when you are
using a flow cell.
SOP Designer NanoPlus Operator’s Manual
3-28 Mar 2014