Particulate Systems NanoPlus, Operator'S Manual, Page: 136 / 252

Maintenance NanoPlus Operator’s Manual
5-22 Mar 2014
Coating the Flat Surface Cell with Polyacrylamide
Read the Vendor’s Material Safety Data Sheet (MSDS) for the Acrylamide before
starting the coating procedure.
You will need the following:
Reagents :
• 3-Methylacryloxypropltrimethoxysilane (organosilane)
• Acrylamide-HG (acrylamide HG)
• N,N,N',N'-Tetramethyl-ethylenediamine (tetramethyl-ethylenediamine)
• Potassium peroxodisulfate
• Methanol
• 0.1 mol/L Sodium hydroxide
• 0.1 mol/L Hydrochloric acid
• Concentrated sulfuric acid
Equipment :
• Drying oven
• Beakers as necessary
To coat the Flat Surface Cell:
1. Immerse the Flat Surface Cell in concentrated sulfuric acid for 2-3 hours. Wash it well with
purified water, and rinse with methanol.
2. Dry the cell in N
2
flow.
3. Place the cell by itself in a drying oven at approximately 160° C. Allow to dry overnight.
Perform steps 4 through 8 in a draft chamber.
4. Remove the cell from the drying oven, and let it stand at room temperature for 15 minutes.
5. Immerse the cell in 0.1 mol/L sodium hydroxide solution for 1.5 hours.
6. Wash the cell with purified water, rinse with methanol, and dry it in N
2
flow.
7. Prepare 200 mL of 60% 3-methylacryloxypropyltrimethoxysilane methanol solution. Filter
the methanol in advance with 0.1-µm filter.