1. Pipet 3 μL of each sample mix into the respective sample inlets on the chip.
2. Pipet 3 μL of each primer mix into the respective primer inlets on the chip.
3. Pipet 150 μL of Actuation Fluid (100-6250) into the P1 reservoir (
) on the chip.
4. Pipet 150 μL of Pressure Fluid (100-6249) into each of the P2 and P3 reservoirs (
) on the chip.
5. Pipet 20 μL of Pressure Fluid into each of the P4 and P5 inlets ( ) on the chip.
6. Place the Olink Signature Q100 Interface Plate (24.192) over the chip, aligning the barcoded edges.
7. Use clear tape to remove any dust particles or debris from the chip surface, if necessary.
IMPORTANT:
Run the chip on Olink Signature Q100 within 60 min of pipetting the samples, primers, and
fluids.
Sample Plate 1
Sample Plate 2
Samples
Primers
Primers
Samples
Primer Plate
Figure 14. Primer and sample pipetting map for the 24.192 chip
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