96.96 chip
48.48 chip
Figure 7. Inserting the syringe into an accumulator
5. Insert the syringe tip into one of the spaces between the arms of the X at the top of the valve and then press down
gently to move the black O-ring to the side. Visually confirm that the O-ring has moved (
).
6. Release the control line fluid:
a. Press the syringe plunger to release the control line fluid into the accumulator while maintaining the 45° angle to
allow the liquid to flow away from the O-ring.
b. Slowly inject the control line fluid by pushing down on the syringe plunger. The control line fluid flows into the
accumulator through the open check valve. Use the entire contents of the syringe.
c. After fully depressing the plunger, wait approximately 5 sec before withdrawing the syringe. Before removing the
syringe from the accumulator, ensure that all the control line fluid and air are purged from the syringe. This is to
avoid dripping fluid on the chip surface. Residual control line fluid or air in the pipette tip is normal.
Syringe tip inserted into
one of the saces between
the arms of the "X".
Move the O-ring to the side.
Syringe tip inserted into
one of the saces between
the arms of the "X".
Move the O-ring to the side.
96.96 chip
48.48 chip
Figure 8. Injecting control line fluid into the chip
7. Check to ensure that the O-ring returns to its normal position after the syringe is removed.
8. Pull the protective film down and away from the bottom of the chip. Discard the film.
9. Place the appropriate (chip-specific) Olink Signature Q100 interface plate over the chip. Align the barcoded edges.
IMPORTANT:
Load the chip within 60 min of completing chip preparation.
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