Notched A1 corner
Figure 12. Inserting the syringe into the accumulator. Note the position of the notched A1 corner.
5. Use the syringe tip to press down gently on the black O-ring to move it (
). Visually confirm that the O-ring has
moved.
Syringe tip
Press down on the O-ring
Figure 13. Injecting control line fluid into the chip
6. Release the control line fluid:
a. Press the syringe plunger to release the control line fluid into the accumulator while maintaining the 45° angle to
allow the liquid to flow away from the O-ring.
b. Slowly inject the control line fluid by pushing down on the syringe plunger. The control line fluid flows into the
accumulator through the open check valve. Use the entire contents of the syringe.
c. After fully depressing the plunger, wait approximately 5 sec before withdrawing the syringe. Before removing the
syringe from the accumulator, ensure that all the control line fluid and air are purged from the syringe. This is to
avoid dripping fluid on the chip surface. Residual control line fluid or air in the pipette tip is normal.
7. Make sure that the O-ring returns to its normal position after the syringe is removed.
8. Pull the protective film down and away from the bottom of the chip. Discard the film.
4.3.2 Pipet Primers, Samples, and Control Reagents into the 24.192 chip
IMPORTANT:
• Vortex all primers, samples, and control reagents thoroughly, then centrifuge them to collect contents before
removing the plate seals and pipetting the mixes into the chip inlets. Failure to do so may result in a decrease in
data quality.
• While pipetting, do not go past the first stop on the pipette. Doing so may introduce air bubbles into inlets.
• Follow the best practices for preventing bubbles, see
.
Refer to
when pipetting the final sample and primer mixes and
when pipetting the control reagents
(Actuation Fluid, Pressure Fluid) into the chip. Note the orientation of the A1 corner. The barcoded edge is on the left side.
NOTE:
Olink recommends using the reverse pipetting method when pipetting the sample and primer mixes. See
the Olink
®
Target 96 User Manual (Olink 0935) or the Olink
®
Target 48 User Manual (Olink 1141).
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