4. Olink Signature Q100
Workflows
4.1 Workflow Overview
Step 1
Step 2
Step 3
Step 4
Step 5
Prepare primers
and samples.
Target 48 and 96:
Prepare the chip
and load primers
and samples.
Focus: Load primers
and samples.
Target 48 and 96:
Run the chip and
collect data on
Olink Signature
Q100.
Focus: Prepare
and run the chip
and collect data
on Olink Signature
Q100.
Transfer the run
data using a USB
drive or directly
import data from
the NPX Signature
software.
Analyze data
using the NPX
Signature
software.
Step Descriptions for 48.48 and 96.96 chips
Workflow Step
1
Prepare the primer and sample mixes.
NOTE:
Do not pipet the samples into the chip at this stage.
2
Inject control line fluid into the accumulators on the chip.
NOTE:
Remember to remove the protective film at this stage.
Prepare the chip on Signature Q100. This primes the chip ready for use.
Remove the chip from the instrument and pipet each final sample and primer mix into the chip.
3
Run the chip and collect data on Olink Signature Q100.
4
Transfer the run data using a USB drive or directly import data from the NPX Signature software.
5
Analyze data using the NPX Signature software.
Step Descriptions for 24.192 chips
Workflow Step
1
Prepare the primer and sample mixes.
NOTE:
Do not pipet the samples into the chip at this stage.
2
Inject control line fluid into the top accumulator on the chip.
NOTE:
Remember to remove the protective film at this stage.
Pipet each final sample and primer mix and the control reagents into the chip.
3
Run the chip and collect data on Olink Signature Q100.
NOTE:
Preparing and running the 24.192 chip occurs in 1 step.
4
Transfer the run data using a USB drive or directly import data from the NPX Signature software.
5
Analyze data using the NPX Signature software.
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