NanoPhotometer
®
NP80/N60/N50/C40
User Guide Version 3.1
41
use a slightly wet lint-free laboratory wipe (with water or 70% EtOH depending on sample
type) to thoroughly clean the sample surface.
•
It is mandatory that the metal contact face around the measurement window and the mirror
is clean.
Cuvette Methods
•
The cuvette holder (NP80/C40 only) is compatible with standard 10 mm path length quartz,
glass and plastic cuvettes with an optical height of 8.5 mm.
•
It is also possible to use cuvettes with 5 mm, 2 mm, 1 mm or 0.5 mm path lengths, but there
may be an adapter necessary. Please ask your cuvette supplier for a suitable adapter.
•
The minimum volume for accurate measurements depends on the cuvette type used; it is
necessary that the light passes through the sample for accurate measurements. Center
height is 8.5 mm.
Note
: The cuvette holder is not removable. Do not pour any cleaning solution into the cuvette
holder as larger amounts of liquids can get into the instrument and cause damage.
Solvent Compatibility (N50/N60/NP80/C40 with submicroliter cell)
Most solvents typically used in life science laboratories are compatible with the
NanoPhotometer
®
nanovolume sample surfaces. The following solvents are compatible for use
with the NanoPhotometer
®
models N50, N60, and NP80 at room temperature:
•
Acetone (
≤
5%)
•
Acetonitrile
•
Benzene
•
Butanol
•
Carbon tetrachloride
•
Chloroform
•
Ethanol
•
Ether
•
HEPES
•
Hexane
•
Isopropanol
•
MES
•
Methanol
•
Methylene chloride
•
MOPS
•
Phenol (
≤1%)
•
N-propanol
•
Toluene
•
Phosphate containing buffers
•
PBS (pH 4-10)
•
Citrate
•
Borate
•
Chloride salts
•
Acids
>
pH 2
•
Bases
<
pH 10
