NanoPhotometer
®
NP80/N60/N50/C40
User Guide Version 3.1
86
In this method, the concentration of the sample is calculated based on the Beer-Lambert law
given the user specified wavelength of interest and user defined extinction coefficient. The
equations for calculating concentration without background correction are as follows:
Without background correction:
𝐶𝐶 = 𝐴𝐴
𝑛𝑛
∗ ε ∗ Ð
C
Concentration (ng/µl)
𝐴𝐴
𝑛𝑛
Absorbance at user specified path length n (10 mm path)
Ð
R
Dilution factor
ε
extinction coefficient/factor
M
ORE
A
PPS
:
S
TANDARD
C
URVE
M
ETHOD
O
VERVIEW
The construction of a calibration curve from multiple standards of known concentrations can be
created and stored on the NanoPhotometer
®
. The standard curve can be used to quantify
samples of the same type with unknown concentrations. This application provides an extremely
useful tool with which to integrate, expedite and simplify the measurement and calculations
involved in determining the concentration of analytes in unknown samples. If a zero
concentration standard is required, include it in the number of standards to be entered using a
reagent blank and entering 0.00 for concentration.
M
EASUREMENTS
P
ROTOCOL
1. Select the More Apps icon from the home screen and the Standard
Curve icon from the More Apps screen.
2.
To change between NanoVolume and Cuvette application, use the
Change to Cuvette/NanoVolume button below the parameter area
(NP80/C40 only).
3.
For NanoVolume application:
Select the dilution depending on the sample concentration
Note
: There is no automatic path length setting in this method. Select either a virtual
dilution of 15 (path length 0.67 mm) or of 140 (path length 0.07 mm).
