NanoPhotometer
®
NP80/N60/N50/C40
User Guide Version 3.1
30
B
UTTONS
Prior to any sample measurement at the start of a new method a blank
measurement of either water or the buffer of the samples is required to give
the NanoPhotometer
®
a reference of what zero should be. It is
recommended to re-apply the blank and measure it as a sample to ensure
the graph of the blank spectra is a flat line.
To initiate the spectral scan of the sample press the sample button. The
data will be temporarily stored until the method is exited; at this time the
user needs to define if the samples should be saved or deleted.
The Change to Cuvette button (NP80) is located at the bottom of the
parameter window. The change to cuvette/nanovolume button toggles
between nanovolume and cuvette applications. Selecting the Change to
Cuvette button activates the cuvette compartment (opens the sliding cover
and switches the red arrow LED on). Only cuvette measurements are
possible when this mode is selected. Selecting the Change to
NanoVolumebutton while the cuvette port is activated will deactivate the
cuvette compartment (closes the sliding cover and switches the LED off)
and only nanovolume measurements are possible.
Note:
Please make sure that the nanovolume arm is closed during cuvette measurements.
The Change to NanoVolume button (C40) is only shown when submicroliter
cell applications are enabled in the preferences. The Change to
NanoVolume/Cuvette button toggles between cuvette and submicroliter cell
applications. With the NanoVolume submicroliter cell application the
parameter of each method shows a list for lid selection (Lid 5, 10, 50, 100
and 250). Choose the correct lid and the results are automatically
calculated considering the virtual dilution.
