NanoPhotometer
®
NP80/N60/N50/C40
User Guide Version 3.1
37
Note:
Do not overfill the well.
Note:
The low energy red light (LED) illumination can be switched of in Preferences
5. Lower the sample arm and initiate a blank measurement with the blank button
6. Clean the measurement window and mirror on the sample arm with a slightly wet lint-free
tissue. Use water, 70% ethanol or isopropanol if needed.
Note:
Make sure that the metal contact face (around the measurement window and the
mirror) is clean.
Note:
Do not use aggressive solvents such as strong acids or bases or organic solvents at
any time (see also page 41 solvent compatibility). If unsure please
contact
for detailed information about your specific reagent/solvent.
7. It is possible to enter a sample name for each sample in the input window “enter sample
name”.
Note
: Allowed characters are: A…Z a…z 0…9 , . - ( ) @ ! = _ ~ ; [ ] { } ‘
Note:
Blank character is not allowed.
8. Raise the sample arm and pipette the appropriate amount of sample solution onto the
illuminated sample window. Upon completion of measurement raise sample arm, clean the
surfaces and apply the next sample.
Note:
Parameter setting Volume 1 - 2 µl adjusts the path length automatically. The
parameter setting Volume 0.3 µl measures only the 0.07 mm path length for higher
concentrations (dsDNA > 420 ng/µl / BSA > 12.6 mg/ml).
Note
: The sample window must be clean and residual fluff from any cleaning wipe must be
removed for optimum performance.
C
UVETTE
M
EASUREMENT
B
ASICS
(C40/NP80)
The NanoPhotometer
®
(NP80 and C40 only) is compatible with standard cuvettes having an 8.5
mm center height. The light path is indicated with a red status LED arrow for the NP80 model
and with two white arrows for the C40 model.