NanoPhotometer
®
NP80/N60/N50/C40
User Guide Version 3.1
67
For NanoVolume Application:
Select the dilution depending on the sample concentration.
Note
: There is no automatic path length setting available in this method. Select either a
virtual dilution of 15 (path length 0.67 mm) or of 140 (path length 0.07 mm).
For NanoVolume application with submicroliter cell (C40 only):
Select Lid depending on your sample concentration (for concentration ranges of different lids
see page 39 Submicroliter Cell Measurements Basics).
Required sample volume for each lid is listed to the right.
Note:
A new Blank is recommended when changing the dilution lid.
4. If it is desired to heat the sample to 37°C use the toggle switch to turn on
the cell holder heater. When the cuvette holder has reached 37°C the
toggle color changes to green.
Note
: Only available for cuvette applications (NP80 and C40).
5.
Default wavelength (
λ
) is 340 nm but can be changed in the range of
200–900 nm (N50: 200–650 nm), depending on the application.
6. Time settings:
Enter the duration time in minutes over which measurements are to be taken. Possible
range is 1–3000 minutes.
Enter the interval time between measurements in seconds. Possible interval times are 5-
