NanoPhotometer
®
NP80/N60/N50/C40
User Guide Version 3.1
71
For
NanoVolume application with submicroliter cell
(C40 only):
Select Lid depending on your sample concentration (for concentration ranges of different lids
see page 39 Submicroliter Cell Measurements Basics).
Required sample volume for each lid is shown.
Note
: A new Blank is recommended when changing the dilution lid.
4. If it is desired to heat the sample to 37°C use the toggle switch to turn on
cell holder heater. When the cuvette holder has reached 37°C the toggle
color changes to green.
Note: Only available for cuvette applications (NP80 and C40).
5. Default wavelength is 600 nm but the wavelength can be changed in the
range of 200–900 nm (N50: 200–650 nm), depending on the application.
6. Toggle switch cells/ml is disabled as default. Enable cells/ml to get the cells/ml calculated.
Enter the cell specific factor and multiplier (e.g. 1 OD600 = 5 x 10
8
cells/ml)
7. Enter the correction factor to compensate for different optical configurations between the
NanoPhotometer
®
and other instruments.
